Gulf BioTerrorism: A Silent & Deadly Enemy Part II

Pandora's Box of Synthetic Biology

Gulf BioTerrorism: A Silent & Deadly Enemy Part II

Postby linn » 27 Feb 2011, 16:28

Gulf BioTerrorism: A Silent & Deadly Enemy Among Us (Part II)
by Michael Edward for http://blueplague.org

“It’s like any other product,” said Charlie Pajor, a senior manager at the Illinois-based company: “We developed them and we’re protecting our trade secret.” Roughly 20-odd dispersant products exist, he said, and while they have similar types of components, they “have proprietary recipes like Coca-Cola”. (May 5, 2010 archive from deepwaterhorizonresponse.com)

Nalco Targets Growth in China with Opening of New Manufacturing Plant

The expansion includes a recently completed $25 million manufacturing plant in Nanjing. “China is a very important market for Nalco,” said Erik Fyrwald, Nalco Chairman, President and Chief Executive Officer. “These new facilities allow us to provide better services and newer technologies to our customers faster and better than any of our competitors.”

The new 240,000 sq. foot (22,300 square meter) plant in the Nanjing Chemical Industry Park has an annual production capacity of 37,000 metric tons. The existing facility has space to expand to 75,000 metric tons per year and the 15-acre (60,000 square meter) site is designed to allow double that capacity for a potential total production of 150,000 metric tons per year. (Source: March 30, 2009 press release at http://www.nalco.com/ap/news-and-events/280.htm).

For extensive links regarding Nalco, including the excellent article Follow The Data, Forget Conspiracy Theory, go to Nalco Background Information at http://worldvisionportal.org/wvpforum/viewtopic.php?f=53&t=1365.

Geobacillus Thermodenitrificans

Darkness Falls (see video at http://www.youtube.com/watch?v=D3IUzVsUk3c), written by Kyle Mills and published by Vanguard Press in 2007, is a science fiction thriller about an eco-terrorist group attempting to destroy the world’s oil supply with synthetically engineered oil-eating super bacteria. “Thriller writers pump up threats to make them bigger and scarier than maybe they really would be. In this case, the opposite is true“. Mills based his book on the bacteria Geobacillus thermodenitrificans that was first discovered and then genetically isolated in 2004 from an oilfield in Daqing, China's largest oil field.

At the same time Darkness Falls was published in 2007, an engineered genetic strain of Geobacillus thermodenitrificans, named NG80-2, was patented and published by the Chinese. This bio-engineered strain uses crude oil as its only carbon source and can degrade 16 to 36 different carbon alkanes (hydrocarbons).

Bacillus Cereus DQ01 & DQ02

These same Chinese scientists responsible for the NG80-2 genetically engineered strain of Geobacillus thermodenitrificans have published numerous reports and World Intellectual patents from 2007 – 2009 for other genetically isolated strains of bacteria that munch on hydrocarbons. These scientists – Hong-Qi Wang and Yan-Jun Chen of the Beijing Normal University and Bo-Ya Qin from the Chinese Ministry of Environmental Protection – also genetically isolated Bacillus cereus DQ01 from soil at the Dagang oil field in northern China. These synthetically engineered microbes are applied to a contamination site or spill in large but controlled volumes and digest their way through the oil, multiplying and digesting until no pollutant remains. They also point out in their papers that adding a small amount of a surfactant material, rhamnolipid, will stimulate enzyme production and greatly improve degradation efficiency.

In other words, these genetically engineered new strains of bacteria the Chinese have created multiply and eat up oil so efficiently that they only stop when their hydrocarbon food source is completely gone. It’s equally important to note that these syn-bio bacteria have to be applied in controlled volumes. No doubt! If too many were applied to an area with access to an oil reservoir, they would eat at it until there was nothing left.

They pointed out that the degradation of crude oil components by Bacillus cereus DQ01 and Bacillus cereus DQ02 were increased 8.1% and 11.6%, respectively, within 48 hours in the presence of the rhamnolipids. They further stated how reproductive growth of the two strains, as well as BATH (bacterial adherence to hydrocarbons) characteristics, greatly increased in the presence of the rhamnolipids. The BATH of Bacillus cereus DQ02 increased 44% in the presence of rhamnolipids.

Why Rhamnolipids?

Rhamnolipids are used as organic solvents for dispersing oil spills. In the studies the Chinese cited, they are used with microorganisms to predominantly remove oil slicks produced by boats and oil spills. They are also used for MEOR –Microorganism Enhanced Oil Recovery. See my It’s Not Wise to Fool Mother Nature article at http://worldvisionportal.org/wordpress/index.php/2010/10/the-gulf-blue-plague-its-not-wise-to-fool-mother-nature/ for MEOR background information.

Rhamnolipids are surfactants, or oil dispersants, and they are not restricted by the U.S. Environmental Protection Agency (EPA). How interesting.

Rhamnolipids are secreted from Pseudomonas aeruginosa, a bacterium. In case you don’t remember the significance of Pseudomonas, let me quote Dr. Riki Ott once again:

“To make things a little scarier, some of the oil-eating bacteria have been genetically modified or otherwise bio-engineered to better eat the oil – including Alcanivorax borkumensis and some of the Pseudomonas.” — September 17, 2010; Bio-Remediation or Bio-Hazard? Dispersants, Bacteria and Illness in the Gulf at http://www.huffingtonpost.com/riki-ott/bio-remediation-or-bio-ha_b_720461.html.


Pseudomonas aeruginosa is a common bacterium which can cause disease in animals, including humans. The symptoms of such infections are generalized inflammation and sepsis. If such colonizations occur in critical body organs, such as the lungs, the urinary tract, and kidneys, the results can be fatal. It is also able to decompose hydrocarbons and has been used to break down tarballs and oil from oil spills.

Blood Poisoning – Septicemia

All I can say is this Chinese developed formula of synthetically engineered bacteria – along with derivatives of other bacteria and surfactants – is already becoming a very dangerous brew, especially with regards to the proven Horizontal Gene Transfer (HGT) characteristics of synthetic bacteria strains.

So now we have an engineered genetic bacterial mix that is a super-charged oil dispersing and oil munching creation. But it appears that it also has a serious down side when it comes to most living creatures, including mankind. They’re using components that are known to cause inflammation and sepsis, just for starters.

A lay term for sepsis is blood poisoning; and whenever there is sepsis, you can expect septicemia. Severe sepsis can easily cause organ dysfunction and hypoperfusion (decreased blood flow through an organ). Septicemia is the presence of severely destructive microorganisms or their toxins in the bloodstream; characterized by chills, fever, prostration (extreme exhaustion), hypotension (abnormally low blood pressure), headache, or pain.

Hemorrhagic Septicemia

As if that’s not bad enough, bacterial hemorrhagic septicemia is usually restricted to system-wide infections by bacteria opportunists such as Pseudomonas. [It's difficult not to notice how the same names keep coming up the further these hydrocarbon eating ingredients and their resulting symptoms are defined]. Bacterial hemorrhagic septicemia is transmitted via contaminated fresh water – rivers, lakes, and ponds - as well as by salt water or by diseased fish from either one.

In fish, vibrio septicemia is a hemorrhage in the skin of the tail and fins; ulceration of the skin; and/or hemorrhage in the muscles and serosal (ie, Gastrointestinal) surfaces. The spleen may be enlarged and bright red. With microscopic study of the cells and tissue, necrosis (cellular death) of the liver, kidney, and spleen are common. Small cutaneous (skin) ulcers and hemorrhaging can be seen in both the skin and muscle. The lesions often cause ulcerative dermatitis. There is also swelling of the liver, spleen and kidneys along with cellular death (necrosis) of the affected tissue with abundant colonies of bacteria.

Vibrio Septicemia is transmitted by contact with diseased fish or contaminated water. The bacteria persist not only in fish, but in the birds that eat them. Perhaps now we have an explanation for why both fresh and salt water fish, as well as birds, are experiencing mass die-offs.

My question is why do all the symptoms found in the fish and birds also show up in Gulf of Mexico residents and cleanup workers?

If there was any parallel argument that Horizontal Gene Transfer (HGT) is occurring here in the Gulf of Mexico, this would be a prime candidate.

Cattle can have hemorrhagic septicemia and there are many diseases classified as both bacterial and viral hemorrhagic septicemia. My point is that this is not just a fish or bird problem. It also effects mammals. Proof is the hemorrhagic septicemia evident with the Dolphins and whales dying in the Gulf of Mexico since May 2010. After all, both dolphins and whales are mammals.

Is this just due to bacteria, or has HGT become a viral factor using genetically engineered bacteriophages (viruses) – that normally attack bacteria – as the method to transfer synthetic DNA into the natural bacteria?

Viral hemorrhagic fever – a group of illnesses caused by a viral infection. Fever and gastrointestinal symptoms are followed by capillary hemorrhage.

Ebola hemorrhagic fever – a severe and often fatal disease in humans and nonhuman primates caused by the Ebola virus; characterized by high fever and severe internal bleeding; can be spread from person to person.

The symptoms don’t lie. Be it viral or bacterial or a combination of both, the synthetically engineered microorganisms introduced into the Gulf of Mexico to eat up the oil and the gas are crossing species barriers. All life from plankton to whales to humans are experiencing the same problems.

The disgusting TV and radio ads still continue in north America. The governments want everyone to come on down to the Gulf beaches; swim in our water; eat our seafood.

I can’t help but think that if there was a way to make the oil and gas disappear along with people, this would be the perfect formula.

“Wherever the Gulf Wind Blows and the Gulf Water Flows”



Additional Sources

Feng L., Wang W., Cheng J., Ren Y., Zhao G., Gao C., Tang Y., Liu X., Han W., Peng X., Liu R., Wang L. ; “Genome and proteome of long-chain alkane degrading Geobacillus thermodenitrificans NG80-2 isolated from a deep-subsurface oil reservoir”; Proc. Natl. Acad. Sci. U.S.A. 104:5602-5607(2007).

“Degradability of n-hexadecane by Bacillus cereus DQ01 isolated from oil contaminated soil from Daqing oil field, China” in Int. J. Environment and Pollution, 2009, 38, 100-115.

Huan Jing Ke Xue. 2007 Sep;28(9):2117-22. Chen YJ, Wang HQ, Wang R, Yun Y. Key Laboratory for Water and Sediment Sciences of Ministry of Education, College of Water Sciences, Beijing Normal University, Beijing 100875, China.

A. Y. Itah and J. P. Essien, Growth Profile and Hydrocarbonoclastic Potential of Microorganisms Isolated from Tarballs in the Bight of Bonny, Nigeria, World Journal of Microbiology and Biotechnology, Volume 21, Numbers 6-7, October, 2005, doi 10.1007/s11274-004-6694-z, p 1317-1322

“Bacterial Diseases of Fish” by Robert B. Moeller Jr., DVM. California Animal Health and Food Safety Laboratory System; University of California

http://www.ncbi.nlm.nih.gov/pubmed/17990568

http://answers.yahoo.com/question/index?qid=20080603022234AALS827

http://www.huffingtonpost.com/riki-ott/bio-remediation-or-bio-ha_b_720461.html

http://en.wikipedia.org/wiki/Pseudomonas_aeruginosa

http://medical-dictionary.thefreedictionary.com/vibrio

http://www.thefreedictionary.com/viral+hemorrhagic+fever
linn
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